[1]谭继翔,何进,秦文熠,等.槲皮素通过抑制TLR4/NF-κB通路缓解脂多糖诱导的急性肾损伤[J].南方医科大学学报,2019,(05):598.[doi:10.12122/j.issn.1673-4254.2019.05.16]
点击复制

槲皮素通过抑制TLR4/NF-κB通路缓解脂多糖诱导的急性肾损伤()
分享到:

《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2019年05期
页码:
598
栏目:
出版日期:
2019-05-15

文章信息/Info

Title:
Quercetin alleviates lipopolysaccharide-induced acute kidney injury in mice by suppressing TLR4/NF-κB pathway
作者:
谭继翔何进秦文熠赵林
关键词:
槲皮素急性肾损伤脂多糖炎症因子TLR4/NF-κB信号通路
Keywords:
quercetin acute kidney injury lipopolysaccharide inflammatory factors TLR4/NF-κB signaling pathway
DOI:
10.12122/j.issn.1673-4254.2019.05.16
摘要:
目的分析槲皮素对脂多糖诱导小鼠急性肾损伤的保护作用,并初步探讨其机制。方法将40 只BALB/c小鼠随机分 为4 组:对照组(无脂多糖及槲皮素),脂多糖组(15 mg/kg 脂多糖),低剂量组(25 mg/kg 槲皮素+15 mg/kg 脂多糖),高剂量组 (50 mg/kg槲皮素+15 mg/kg脂多糖)。槲皮素予以胃灌注预处理,1次/d,连续3 d,对照组同时予以相同体积的生理盐水。最后 1次灌胃后1 h予以腹腔注射15 mg/kg的脂多糖,脂多糖干预24 h后结束实验,处死小鼠。观察小鼠肾脏组织病理变化和血肌 酐、尿素氮评估肾脏损伤情况,ELISA 法检测血清中TNF-α、IL-1β、IL-6 的表达情况,Western blot 检测肾脏胞浆中TLR-4、 MyD88、TRAF-6以及核内NF-κB p65蛋白的表达。结果槲皮素可以降低脂多糖诱导的急性肾损伤的肌酐、尿素氮水平以及肾 脏损伤的评分(P<0.05),降低血清中TNF-α、IL-1β、IL-6的表达(P<0.05),槲皮素抑制肾脏组织中TLR4、MyD88、TRAF-6及核 内NF-κB p65蛋白的表达(P<0.05)。结论槲皮素预处理可能通过抑制TLR4/NF-κB信号通路保护脂多糖诱导的小鼠急性肾损伤。
Abstract:
Objective To investigate the protective effect of quercetin against lipopolysaccharide (LPS)- induced acute kidney injury (AKI) in mice and explore its mechanism. Methods Forty male BALB/c mice were randomly divided into control group (with saline treatment), 15 mg/kg LPS group, and quercetin-treated groups with intragastric quercetin treatment (once daily for 3 consecutive days) at low (25 mg/kg) and high (50 mg/kg) dose prior to 15 mg/kg LPS injection. LPS was administered by intraperitoneally injection 1 after the last gavage of quercetin. The mice were sacrificed 24 h after LPS injection for analysis of kidney pathologies, blood urea nitrogen (BUN) and creatinine levels; serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 were detected by ELISA, and the expressions of Toll-like receptor-4 (TLR4), MyD88, TRAF-6 and NF-κBp65 in the kidney were detected by Western blotting. Results Quercetin significantly lessened renal pathologies, lowered BUN and creatinine levels (P<0.05) and inhibited TNF-α, IL-1β, and IL-6 production in mice with LPS-induced AKI (P< 0.05). Pretreatment with quercetin also significantly inhibited TLR4, MyD88, and TRAF-6 expressions and NF-κBp65 activation in the kidneys of the rats with LPS challenge (P<0.05). Conclusion Quercetin pretreatment can protect mice against LPSinduced AKI by inhibiting TLR4/NF-κB signaling pathway.

相似文献/References:

[1]张兰兰,曹琼,胡子有,等.槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区神经干细胞增殖的影响[J].南方医科大学学报,2011,(07):1200.
[2]苑召虎,胡子有,张兰兰,等.槲皮素对胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响[J].南方医科大学学报,2013,(02):207.
[3]张颖,刘宁,任青爱,等.硫氢化钠防治截肢术后大鼠肾脏损伤的机制[J].南方医科大学学报,2013,(08):1146.
[4]王惠丽,苑召虎,陈志江,等.槲皮素诱导U87细胞凋亡及对MDM2-p53负反馈调节的影响[J].南方医科大学学报,2014,(05):686.
[5]李坚,张剑,董欣敏,等.槲皮素对内毒素性心肌损伤的保护作用及机制[J].南方医科大学学报,2015,(07):1068.
[6]雷莹,聂晟,孙丹华,等.中国危重症住院患者急性肾损伤的流行病学分析[J].南方医科大学学报,2016,(06):744.
[7]吴光勇,庄恺,刘宇,等.尿肝型脂肪酸结合蛋白在颅脑外伤中应用价值[J].南方医科大学学报,2016,(11):1527.
[8]霍锐,戴敏,樊艺,等.miRNA-29a和miRNA-10a-5p 对脓毒症所致急性肾损伤患者28 d 死亡率的预测价值[J].南方医科大学学报,2017,(05):646.
[9]梁青春,陈燕亭,李传翔,等.槲皮素调节ROS/TLR4信号通路抑制Ox-LDL诱导的血管平滑肌细胞钙化[J].南方医科大学学报,2018,(08):980.
[10]刘崇斌,黄柳维,李彩珍,等.阻断Pannexin-1 可减轻肾脏组织炎症细胞浸润和缓解顺铂诱导的急性肾损伤[J].南方医科大学学报,2019,(05):508.[doi:10.12122/j.issn.1673-4254.2019.05.02]

更新日期/Last Update: 1900-01-01