[1]陈永和,苏宝倡,尚孟乔.P2X7受体在类风湿关节炎中的诊断价值及炎症反应中的作用[J].南方医科大学学报,2018,(12):1453.[doi:10.12122/j.issn.1673-4254.2018.12.09]
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P2X7受体在类风湿关节炎中的诊断价值及炎症反应中的作用()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年12期
页码:
1453
栏目:
出版日期:
2018-12-31

文章信息/Info

Title:
Diagnostic value of P2X7 receptor and its role in inflammatory reaction in rheumatoid arthritis
作者:
陈永和苏宝倡尚孟乔
关键词:
类风湿关节炎P2X7受体炎症细胞因子
Keywords:
rheumatic arthritis P2X7 receptor inflammatory factor
DOI:
10.12122/j.issn.1673-4254.2018.12.09
摘要:
目的研究P2X7受体在类风湿关节炎中诊断价值及在炎症反应中作用。方法以骨关节置换病人滑膜组织为对照组(n= 25),类风湿关节炎患者滑膜组织为病例组(n=25),qRT-PCR法检测骨关节置换病人和类风湿关节炎患者滑膜组织中P2X7受体 相对表达;特异性敲低P2X7受体的小分子干扰RNA干扰P2X7受体研究其对炎症细胞因子分泌作用;ELISA检测炎症细胞因 子白细胞介素IL-1β、IL-6、IL-8水平。结果类风湿关节炎患者滑膜组织P2X7受体水平明显高于骨关节置换手术患者滑膜组织 P2X7受体水平,其对类风湿关节炎诊断具有较好的价值;类风湿关节炎患者滑膜组织P2X7受体水平与血清中炎症细胞因子 IL-1β、IL-6、IL-8水平呈正相关,且有统计学意义(P<0.001);干扰P2X7受体后明显抑制MH7A滑膜细胞的IL-1β和IL-6分泌。 结论类风湿关节炎患者滑膜组织P2X7受体水平升高,对类风湿关节炎具有诊断价值,阻断P2X7受体后抑制炎症细胞因子的 分泌和炎症反应。
Abstract:
Objective To study the diagnostic value of P2X7 receptor for rheumatoid arthritis (RA) and its role in the inflammatory response. Methods With the synovial tissues from 25 patients with bone and joint replacement as the control, the synovial tissues of 25 RA patients were examined for the relative expression of P2X7 receptor mRNA using qRT-PCR. In an immortalized RA synovial cell line (MH7A), the effect of P2X7 receptor knockdown via a small interfering RNA were examined on the productions of the inflammatory cytokines including interleukin-1β (IL-1β), IL-6, and IL-8 using ELISA. Results The RA patients showed significantly higher levels of P2X7 receptor mRNA expression in the synovial tissue than the control patients. P2X7 receptor had a good diagnostic value for RA. The expression levels of IL-1β, IL-6, and IL-8 were positively correlated with the levels of P2X7 receptor in the synovial tissues of RA patients (P<0.001). In MH7A cells, P2X7 receptor knockdown obviously reduced the secretion of IL-1β and IL-6. Conclusion RA patients show elevated P2X7 receptor level in the synovial tissue, which has a good diagnostic value for RA. Blocking P2X7 receptor can inhibit inflammatory factor secretion and suppress inflammatory reactions.

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更新日期/Last Update: 1900-01-01