[1]李娴,李姗姗,王新新,等.敲低Fascin抑制宫颈癌细胞增殖及裸鼠的成瘤能力[J].南方医科大学学报,2018,(12):1409.[doi:10.12122/j.issn.1673-4254.2018.12.02]
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敲低Fascin抑制宫颈癌细胞增殖及裸鼠的成瘤能力()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年12期
页码:
1409
栏目:
出版日期:
2018-12-31

文章信息/Info

Title:
Knocking down fascin inhibits cervical cancer cell proliferation and tumorigenesis in nude mice
作者:
李娴李姗姗王新新赵素容刘浩
关键词:
Fascin宫颈癌裸鼠成瘤细胞增殖
Keywords:
fascin cervical cancer tumorigenesis cell proliferation
DOI:
10.12122/j.issn.1673-4254.2018.12.02
摘要:
目的研究敲低Fascin对宫颈癌细胞增殖及裸鼠成瘤能力的影响。方法Fascin siRNA慢病毒(干扰组)和阴性对照慢病 毒(阴性组)感染宫颈癌CaSki细胞,用qRT-PCR和Western blot方法检测Fascin mRNA以及蛋白表达水平评价干扰效果。MTT 方法测定Fascin 敲低对CaSki细胞增殖能力的影响。取对照组、阴性组、干扰组CaSki细胞接种至裸鼠皮下,建立裸鼠移植瘤模 型,测定移植瘤生长体积和质量,Western blot方法检测移植瘤组织中增殖细胞核抗原(PCNA)、survivin、细胞周期依赖性蛋白 激酶4(CDK4)、p21 蛋白水平。结果Fascin siRNA慢病毒感染后的CaSki 细胞中Fascin mRNA和蛋白水平低于没有感染的 CaSki细胞(P<0.05)。阴性对照慢病毒感染对CaSki细胞中Fascin mRNA和蛋白水平没有影响。敲低Fascin后的CaSki细胞增 殖能力明显降低(P<0.05)。干扰组CaSki接种的裸鼠移植瘤体积和质量也降低,移植瘤组织中PCNA、survivin、CDK4蛋白水平 降低,p21蛋白水平升高。阴性组CaSki细胞接种对细胞增殖、裸鼠成瘤能力及移植瘤中PCNA、survivin、CDK4、p21蛋白水平 没有影响。结论敲低Fascin 能够抑制宫颈癌细胞生长和裸鼠成瘤能力。
Abstract:
Objective To study the effect of knocking down fascin on cervical cancer cell proliferation and tumorigenicity in nude mice. Methods Cervical cancer CaSki cells were infected with a lentiviral vector carrying fascin siRNA or with a negative control lentivirus, and fascin mRNA and protein expressions in the cells were detected using qRT-PCR and Western blotting. MTT assay was used to determine the proliferation of CaSki cells with fascin knockdown. CaSki cells transfected with fascin siRNA or the control lentiviral vector and non-transfected CaSki cells were inoculated subcutaneously in nude mice, and the volume and weight of the transplanted tumor were measured; Western blotting was used to detect the expressions of proliferating cell nuclear antigen (PCNA), survivin, cyclin dependent kinase 4 (CDK4) and p21 proteins in the tumor xenograft. Results Infection with the lentiviral vector carrying fascin siRNA, but not the negative control vector, caused significant reductions in the expression levels of fascin mRNA and protein in CaSki cells (P<0.05). Fascin knockdown resulted in significantly reduced proliferation of CaSki cells in vitro (P<0.05). The nude mice inoculated with CaSki cells with fascin knockdown showed reduced tumor volume and weight, lowered levels of PCNA, survivin and CDK4, and increased expression of p21 protein in the tumor xenograft compared with the control mice. The negative control lentivirus did not affect the proliferation or tumorigenicity of CaSki cells in nude mice or the expression levels of PCNA, survivin, CDK4 or p21 proteins in the xenografts. Conclusion Knocking down fascin can inhibit the growth and tumorigenicity of cervical cancer cells in nude mice.

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更新日期/Last Update: 1900-01-01