[1]韩跃峰,陈德尚,李慧,等.长链非编码RNA MALAT-1基因的敲除抑制人喉鳞状细胞癌Hep-2细胞的增殖和迁移[J].南方医科大学学报,2018,(08):923.
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长链非编码RNA MALAT-1基因的敲除抑制人喉鳞状细胞癌Hep-2细胞的增殖和迁移()
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《南方医科大学学报》[ISSN:1673-4254/CN:44-1627/R]

卷:
期数:
2018年08期
页码:
923
栏目:
出版日期:
2018-07-31

文章信息/Info

Title:
Long chain non-coding RNA MALAT-1 gene knockdown inhibits growth and migration and promotes apoptosis of human laryngeal squamous cell carcinoma Hep-2 cells in vitro
作者:
韩跃峰陈德尚李慧王晓敏张明洁杨洋
关键词:
细胞凋亡长链非编码RNA喉肿瘤MALAT1迁移和侵袭
Keywords:
apoptosis long chain non coded RNA laryngeal neoplasms MALAT1 migration and invasion
摘要:
目的探讨敲除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的影响。方法使用实时聚合酶链反应(RTPCR) 以永生化鼻咽上皮(NPE)细胞株NP-69作为参照检测FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,使用shmalat1 慢病毒转染Hep-2细胞,RT-PCR检测其MALAT1的表达。增殖速率分析,MTT法明确MALAT-1对细胞增殖的影响。流式细 胞术分析细胞周期和细胞凋亡。采用Transwell 小室检测Hep-2 细胞的迁移。最后使用Matrigel 侵袭实验评估shmalat1 和 shCtrl慢病毒转染的Hep-2细胞的侵袭能力。结果使用qPCR检测FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,结果 发现与NP-69细胞相比,FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达显著增加。敲除MALAT1明显抑制Hep-2细胞增 殖。与MOCK和shCtrl 细胞相比,MALAT1-shRNA 慢病毒转染细胞S期细胞数量显著增加(P<0.01)。此外,细胞在G2/M期 的百分比下降(P<0.01)。下调MALAT1时,Hep-2细胞的侵袭和迁移都受到抑制。结论MALAT1在喉鳞状细胞癌高表达。敲 除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的增殖、凋亡、侵袭和迁移起抑制作用。
Abstract:
Objective To investigate the effect of knocking down long chain non-coding RNA MALAT-1 gene on the biological behaviors of human laryngeal squamous cell carcinoma Hep-2 cells. Method With immortalized nasopharyngeal epithelial (NPE) cell line NP-69 as the reference, MALAT1 expression in FaDu, Hep-2 and nasopharyngeal carcinoma CNE-2Z cells were detected using real-time PCR. Hep-2 cells were transfected with shmalat1 lentivirus and the expression of MALAT1 was detected. MTT assay, flow cytometry, Transwell assay and M Atrigel invasiveness test were used to evaluate the effect of MALAT-1 knockdown on the proliferation, cell cycle, cell apoptosis, migration, and invasiveness of Hep-2 cells. Results Compared with NP-69 cells, Hep-2 cells, FaDu cells, and CNE-2Z cells all showed significantly increased MALAT-1 expression. In Hep-2 cells, knockdown of MALAT-1 significantly inhibited the cell proliferation, increased the cell percentage in S phase (P<0.01), decreased the cell percentage in G2/M phase (P<0.01), and attenuated the migration and invasiveness of the cells. Conclusion MALAT-1 is over-expressed in laryngeal squamous cell carcinoma, and knocking down MALAT-1 gene can significantly suppress the proliferation, invasion and migration and promotes apoptosis of the cancer cells.

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更新日期/Last Update: 1900-01-01