[1]李双凤,欧阳碧山,赵鑫,等.右美托咪定对奥沙利铂致神经性疼痛的镇痛作用及机制[J].南方医科大学学报,2018,(07):836.
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右美托咪定对奥沙利铂致神经性疼痛的镇痛作用及机制()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年07期
页码:
836
栏目:
出版日期:
2018-07-16

文章信息/Info

Title:
Analgesic effect of dexmedetomidine on oxaliplatin-induced neuropathic pain in rats
作者:
李双凤欧阳碧山赵鑫王亚平
关键词:
右美托咪定奥沙利铂神经病理性疼痛p-STAT3
Keywords:
dexmedetomidine oxaliplatin neuropathic pain p-STAT3
摘要:
目的探讨右美托咪定(Dex)对奥沙利铂(Oxa)致神经性疼痛的镇痛作用及其机制。方法采用单剂量Oxa腹腔注射诱发 大鼠神经性疼痛模型。SD大鼠随机分为4组:空白对照组(control组)、神经性疼痛组(model组)、右美托咪定处理组(Dex组)、 右美托咪定+阿替美唑组(Ati组)。在行为学上检测各组大鼠机械痛阈值(PWT)和冷热痛阈值(TWL);应用免疫印记法和免疫 荧光法检测大鼠脊髓p-STAT3的蛋白表达。结果与control组比较,model组和Ati组PWT和TWL(冷)显著性缩短(P<0.05); 脊髓p-STAT3 的表达水平显著性增加(P<0.01)。与model 组比较,Dex 处理后60~120 min PWT和TWL(冷)显著性延长(P< 0.05);脊髓p-STAT3的表达显著性减少(P<0.01)。与Dex组比较,Ati组在Dex处理后60~120 min PWT和TWL(冷)显著性缩 短(P<0.05),而脊髓p-STAT3的表达显著性增加(P<0.05)。结论Dex可通过抑制大鼠脊髓p-STAT3的增长,缓解Oxa所致的神 经性疼痛。
Abstract:
Objective To investigate the analgesic effect of dexmedetomidine (Dex) on oxaliplatin-induced neuropathic pain in rats and explore its mechanism. Methods SD rats were randomly divided into control, model, Dextreatment, and Dex + atipamezole groups. In the latter 3 groups, rat models of neuropathic pain were established by a single intraperitoneal injection of oxaliplatin. The paw withdrawal threshold (PWT) to mechanical stimuli and tail withdrawal latency (TWL) to thermal stimuli of the rats were determined. Western blotting and immunofluorescence assay were performed to observe the expression of spinal phosphorylated STAT3 (p-STAT3) in the rats. Results Compared with the rats in the control group, the rats in the model group and Dex+atipamezole group showed significantly decreased PWT and TWL (cold) and increased expression of p-STAT3 in the spinal cord (P<0.05). In Dex group, PWT and TWL (cold) were significantly increased (P<0.05) and p-STAT3 expression in the spinal cord was significantly decreased (P<0.01) 60 to 120 after Dex treatment as compared with those in the model group, and these effects of Dex were significantly attenuated by the administration of atipamezole (P<0.05). Conclusion Dex can alleviate oxaliplatin-induced neuropathic pain in rats by inhibiting the phosphorylation of STAT3 in the spinal cord.

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更新日期/Last Update: 1900-01-01