[1]肖汉森,谢茜,钟家禹,等.波形蛋白对EV71感染小鼠脑组织引起NLRP3炎性小体活化的影响[J].南方医科大学学报,2018,(06):704.
点击复制

波形蛋白对EV71感染小鼠脑组织引起NLRP3炎性小体活化的影响()
分享到:

《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年06期
页码:
704
栏目:
出版日期:
2018-06-30

文章信息/Info

Title:
Effect of vimentin on activation of NLRP3 inflammasome in the brain of mice with EV71 infection
作者:
肖汉森谢茜钟家禹Bisanga Gerald Rukundo何肖龙屈雅丽曹虹
关键词:
EV71波形蛋白炎性小体中枢神经系统
Keywords:
EV71 vimentin inflammasome central nervous system
摘要:
目的观察肠道病毒71型(EV71)感染的小鼠中枢神经系统中波形蛋白(VIM)介导NLRP3炎性小体的活化。方法取3~ 5 d龄的VIM基因敲除型乳鼠(VIM-/-)40只,随机分为EV71感染实验组和空白对照组,20只/组,实验组每只腹腔注射浓度为1× 108半数组织培养感染剂量(TCID50)病毒液10 μL,空白组腹腔注射无菌PBS 10 μL,再将同品质的野生型乳鼠(WT)40只以同样 方式分组处理。观察小鼠感染状态1周,提取小鼠脑脊液(CSF)和脑组织全蛋白、RNA及组织切片,通过Western blot、ELISA、 RT-PCR检测小鼠中枢神经系统的炎症小体激活状况,以及免疫组化技术考察小鼠中枢神经系统的损伤。结果与空白组比 较,VIM-/-实验组小鼠在感染EV71 后CSF 和脑组织中NLRP3 炎症小体及其下游产物IL-1β、caspase-1 含量无明显变化;而 WT实验组小鼠相较VIM-/-型实验组的NLRP3、IL-1β和caspase-1含量显著升高(P<0.05);同时WT型实验组IL-1β和caspase-1 的mRNA拷贝数亦明显高于VIM-/-型实验组小鼠(P<0.05);VIM-/-感染EV71 的脑组织神经元受损程度较WT小鼠明显轻微 (P<0.05)。结论EV71通过感染小鼠脑组织诱发VIM基因介导的NLRP3炎症小体活化,释放IL-1β和caspase-1,这可能是中枢 神经系统炎症和神经元损伤的原因之一。
Abstract:
Objective To explore whether vimentin (VIM) mediates the activation of inflammasome in mice with EV71 infection in the central nervous system. Methods Forty VIM knockout mice (VIM-/-, 3 to 5 days old) were randomly divided into control group and infection group. The infection group was intraperitoneally injected with EV71 (108 TCID50), while the control group was injected with PBS (10 μL); another 40 wild-type mice (WT, 3 to 5 days old) were grouped in the same manner. The general conditions of mice were observed each day. Western blotting, ELISA, and RT-PCR were used to measure the levels of IL-1β and casepase-1 in the brain or cerebrospinal fluid. The pathological changes in the cerebella and brain were observed using immunohistochemistry. Results Compared with the control group, the VIM-/- mice infected with EV71 showed no significant changes in NLRP3, IL-1β or caspase-1 expression. The WT mice infected with EV71 showed obviously increased NLRP3, IL-1β, and caspase-1 expressions in the central nervous system. The neurons of infected VIM-/- mice exhibited milder cell damage than the those in WT mice. Conclusion VIM mediates the activation of inflammasome and promotes brain inflammation and neuronal damage in mice with EV71 infection in the central nervous system.

相似文献/References:

[1]赵文红,俞慧,张建国,等.草甘膦对小鼠睾丸支持细胞凋亡及雄激素结合蛋白、波形蛋白mRNA表达的影响[J].南方医科大学学报,2013,(11):1709.
[2]黎天尊,晏 怡,刘 强,等.细胞凋亡信号调节蛋白1对大鼠脊髓损伤后GFAP、vimentin表达及后肢运动功能的影响[J].南方医科大学学报,2015,(06):795.
[3]吕建平,曹志恺,Lee Jin-Moo.四波长内源光信号成像在GFAP-/-Vim-/-小鼠脑梗塞周围扩散性去极化中的应用[J].南方医科大学学报,2015,(03):417.
[4]刘春花,江庆萍,林丹,等.子宫内膜癌中丝裂原活化蛋白激酶4和波形蛋白的表达及临床意义[J].南方医科大学学报,2017,(02):157.

更新日期/Last Update: 1900-01-01