[1]耿忆薇,王亚兰,邓蓉,等.疏风活血方通过自噬途径对体外培养的鼠B16黑素瘤细胞黑素代谢起调节作用[J].南方医科大学学报,2018,(05):630.
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疏风活血方通过自噬途径对体外培养的鼠B16黑素瘤细胞黑素代谢起调节作用()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年05期
页码:
630
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Shufeng Huoxue Formula suppresses proliferation and regulates melanin metabolism in murine B16 melanoma cells in vitro through autophagy pathway
作者:
耿忆薇王亚兰邓蓉傅楷历邓燕
关键词:
自噬B16黑素合成酪氨酸酶活性LC3BBeclin1P62p-mTOR
Keywords:
autophagy B16 melanin metabolism tyrosinase activity LC3B Beclin1 P62 p-mTOR
摘要:
目的观察自噬在疏风活血方对体外培养的鼠B16黑素瘤细胞增殖及黑素合成中的作用。方法体外培养鼠B16细胞,设 立空白对照组(C组),0.12、0.25、0.49、0.98、1.96 mg/mL疏风活血方组(S组),自噬诱导剂雷帕霉素组(R组),自噬诱导+疏风活 血方组(RS组),采用MTT法测定各组对B16细胞增殖的影响,酶标仪检测法测定各组酪氨酸酶活性及黑素含量,Western blot 法测定自噬相关蛋白P62、p-mTOR、LC3B、Beclin1含量,比较分析结果。结果与C组相比,S组及RS组对B16细胞增殖均起浓 度依赖的抑制作用(P<0.05);对酪氨酸酶活性及黑素合成有促进作用(P<0.05);0.98 mg/mL疏风活血方组(R0.98)、自噬诱导+ 0.98 mg/mL疏风活血方组(RS0.98)、50 nmol/L自噬诱导剂雷帕霉素组(Rap50)均能上调自噬相关蛋白LC3B-II、Beclin1,下调 P62、p-mTOR含量。结论疏风活血方能通过激活自噬途径对体外培养的鼠B16黑素瘤细胞黑素代谢起调节作用,对其细胞增 殖有抑制作用;对酪氨酸酶活性及黑素合成起促进作用。
Abstract:
Objective To investigate the role of autophagy in the regulatory effect of Shufeng Huoxue Fumula (SFHXF) on the proliferation and melanin metabolism in cultured murine B16 melanoma cells. Methods B16 cells were treated with solutions containing 0.12, 0.25, 0.49, 0.98, or 1.96 mg/mL SFHXF preparations, rapamycin (an autophagy inducer), or rapamycin+SFHXF. The changes in the proliferation of B16 cells were assessed using MTT assay, and tyrosinase activity and melanin content in the cells were determined. The expressions of autophagy- related proteins P62, p-mTOR, LC3B, and beclin 1 in the cells were detected using Western blotting. Result Compared with the blank control cells, treatments with SFHXF both in the presence and in the absence of rapamycin concentration-dependently inhibited the cell proliferation (P<0.05) and obviously increased tyrosinase activity and melanogenesis in B16 cells (P<0.05); 0.98 mg/mL SFHLF, rapamycin+0.98 mg/mL SFHXF, and 50 nmol/L rapamycin all significantly up-regulated the expressions of LC3B-II and beclin 1 and down-regulated the expressions of P62 and p-mTOR in the cells. Conclusion SFHXF can regulate melanin metabolism and enhance tyrosinase activity and melanogenesis through the autophagy pathway to inhibit the proliferation of B16 cells in vitro.

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更新日期/Last Update: 1900-01-01