[1]张韫琪,蔡云,刘媛,等.甘草次酸选择性抑制肝癌细胞的增殖[J].南方医科大学学报,2018,(04):477.
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甘草次酸选择性抑制肝癌细胞的增殖()
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《南方医科大学学报》[ISSN:/CN:]

卷:
期数:
2018年04期
页码:
477
栏目:
出版日期:
2018-04-30

文章信息/Info

Title:
Glycyrrhetinic acid selectively inhibits proliferation of hepatocellular carcinoma cells in vitro
作者:
张韫琪蔡云刘媛赵博欣李国锋
关键词:
甘草次酸肝癌细胞增殖速率拓扑异构酶ⅡαERK通路
Keywords:
glycyrrhetinic acid hepatocellular carcinoma proliferation rate topoisomerase IIα ERK pathway
摘要:
目的探讨甘草次酸对增殖速率不同的4株肝癌细胞SMMC-7721,SK-HEP1,HEPG2,HEP3B体外增殖的抑制作用及其 机制。方法通过MTT法测定肝癌SMMC-7721,SK-HEP1,HEPG2和HEP3B细胞株体外生长曲线;用梯度浓度甘草次酸(5、 10、20、30、40、60 μmol/L)分别刺激48 h后,MTT法检测甘草次酸对4种肝癌细胞株体外生长的影响,Western blot实验考察其对 4种肝癌细胞株总ERK蛋白,磷酸化ERK蛋白和拓扑异构酶Ⅱα蛋白表达的影响。结果4株肝癌细胞中SMMC-7721细胞增殖 速率最慢,而SK-HEP1 细胞增殖速率最快;甘草次酸刺激48 h 能浓度依赖性地抑制4 种肝癌细胞的生长,其中对慢增殖细胞 SMMC-7721 细胞抑制作用最强(IC50为28.04 μmol/L);细胞内ERK的磷酸化,拓扑异构酶Ⅱα的表达与细胞增殖速率呈正相 关,SMMC-7721 细胞拓扑异构酶Ⅱα与p-ERK蛋白的表达在4 株细胞中最低,SK-HEP1 细胞表达最高。与阴性对照组相比, 50 μmol/L的甘草次酸可显著抑制4株细胞内拓扑异构酶Ⅱα与p-ERK蛋白的表达(P<0.05),而25 μmol/L的甘草次酸可显著下 调SMMC-7721,HEPG2和HEP3B细胞拓扑异构酶Ⅱα与p-ERK蛋白(P<0.05)而对SK-HEP1细胞没有作用。结论甘草次酸 对不同肝癌细胞生长的抑制作用有选择性。其中对慢增殖肝癌的治疗具有较好的抑制效果。下调拓扑异构酶Ⅱα的表达与抑 制ERK通路的激活可能为其作用机制之一。
Abstract:
Objective To investigate the selective inhibitory effect of glycyrrhetinic acid on 4 hepatocellular carcinoma (HCC) cells with different proliferation rates and explore the underlying mechanisms. Methods MTT method was used to detect the proliferation rates of 4 HCC cell lines, namely SMMC-7721, SK-HEP1, HEPG2 and HEP3B. Following treatment of the cells with glycyrrhetinic acid (5, 10, 20, 30, 40, and 60 μmol/L), the cell viability was analyzed using MTT assay and the expressions of total ERK protein, p-ERK protein and topoisomerase IIα were detected using Western blotting. Results Among the 4 cell lines, SMMC-7721 had the lowest and SK-HEP1 had the highest proliferation rate. Treatment with glycyrrhetinic acid for 48 h dose-dependently inhibited the proliferation of all the 4 cell lines in vitro and produced the strongest inhibitory effect in SMMC-7721 cells with the IC50 of 28.04 μmol/L. The proliferation rate of the cells was positively correlated with the expression levels of p-ERK and topoisomerase IIα, which were the lowest in SMMC-7721 cells and the highest in SK-HEP1 cells. Treatment with 50 μmol/L glycyrrhetinic acid significantly down-regulated the expressions of p-ERK and topoisomerase IIα in the 4 HCC cell lines (P<0.05), while 25 μmol/L glycyrrhetinic acid significantly reduced the expression of topoisomerase IIα and p-ERK in SMMC-7721, HEPG2 and HEP3B cells (P<0.05) but not in SK-HEP1 cells. Conclusion Glycyrrhetinic acid can inhibit the proliferation of different HCC cells particularly in cells with a low proliferation rate. The inhibitory effect of glycyrrhetinic acid might be mediated by reducing the expressions of topoisomerase IIα and inhibiting the ERK pathway.

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更新日期/Last Update: 1900-01-01